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. 2019 Apr 6;15(12):2091–2106. doi: 10.1080/15548627.2019.1596493

Figure 2.

Figure 2.

Effect of Atg5 deficiency and PtdIns3K inhibitor treatment on CD8+ T-cell priming and MHC class I presentation. (a) Wild-type BMDCs were pre-incubated with LY294002 and then co-cultured with irradiated EG7 cells and OT-I CD8+ T cells. Concentrations of IFNG/IFN-γ in the culture medium were measured by ELISA. Mean concentrations are presented with standard deviations (Student’s t-test; *P < 0.05). Data are representative of 3 similar independent experiments. (b) Atg5± or atg5−/- NLDCs were co-cultured with irradiated EG7 cells and OT-I CD8+ T cells. After 72 h, concentrations of IFNG/IFN-γ in supernatant were measured by ELISA. Data are representative of 3 similar experiments. (c) Atg5+/- or atg5−/- NLDCs were co-cultured with irradiated EG7 cells. After 24 h, SERPINB/OVA-specific MHC class I antigen presentation was measured by antibody for SERPINB/OVA257-264 (SIINFEKL) peptide bound to H-2Kb. (d) Dot graph showing data pooled from 4 independent experiments. Error bars indicate the standard error of the mean.