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. 2019 Mar 30;15(11):1954–1969. doi: 10.1080/15548627.2019.1596486

Figure 2.

Figure 2.

Cerulein decreases acinar cell lysosome numbers and induces insufficient autophagy in mouse pancreas. Male GFP-LC3 transgenic mice were administrated with 7 hourly intraperitoneal injections of cerulein (50 μg/kg). Cryo-sections of pancreas were subjected to immunostaining for LAMP1 and nuclei were stained with Hoechst33342. (a) Representative confocal microscopy images of cryo-sections of pancreas are shown. Arrows represent colocalized puncta of GFP-LC3 with LAMP1. Arrowheads indicate green-only GFP-LC3 puncta. Bar: 20 µm. (b) The numbers of GFP-LC3 puncta, LAMP1 puncta and GFP-LC3/LAMP1 overlay puncta per cell in each group were quantified. More than 120 cells were counted in each mouse. Data are mean ± SE (n = 4). **p < 0,01; Student t-test analysis. (c) Male GFP-LC3 transgenic mice were administrated with chloroquine (CQ, 60 mg/kg, i.p) or saline followed by 7 hourly intraperitoneal injections of cerulein (50 μg/kg) or saline. Total pancreatic lysates were subjected to immunoblotting analysis. Densitometry analysis were performed for SQSTM1 and LC3-II (normalized to loading control GAPDH) and data shown are mean ± SE (n = 3–4).