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. 2019 Aug 30;16(12):1697–1710. doi: 10.1080/15476286.2019.1656026

Figure 3.

Figure 3.

Suppression of siRNA pathway by viral synonymous SNP in insect cells. (a) Influence of synonymous SNP of wsv226 mRNA on siRNA pathway in insect cells. The insect High Five cells were co-transfected with wild-type siRNA (WT siRNA), SNP siRNA, EGFP-WT-mRNA and/or EGFP-SNP-mRNA of wsv226. At 36 h after co-transfection, the wsv226 mRNA level was examined by Northern blot. U6 was used as a control. Probes were indicated on the left. (b) Evaluation of viral synonymous SNP of wsv226 mRNA on siRNA pathway in insect cells by examination of fluorescence intensity of insect cells. The treatments were indicated on the top. Scale bar, 50 μm. (c) Role of synonymous SNP of wsv151 mRNA in siRNA pathway in insect cells. At 36 h after co-transfection of insect cells with wild-type (WT) siRNA, SNP siRNA, EGFP-WT-mRNA and/or EGFP-SNP-mRNA of wsv151, the wsv151 mRNA level was determined by Northern blot. U6 was used as a control. Probes were indicated on the left. (d) Impact of viral synonymous SNP of wsv151 mRNA on siRNA pathway in insect cells by examination of fluorescence intensity of insect cells. The treatments were indicated on the top. Scale bar, 50 μm.