Evolutionary conservation of the short ribosomal ramp. (A) Density histogram of GFP scores of genes identified in E. coli (Escherichia), E. asburiae (Enterobacter), K. oxytoca (Klebsiella) and Homo sapiens for nucleotide compositions at codon positions 3–5. As a control, the GFP library score was plotted. (B) A list of 1,595 orthologous genes of E. coli, E. asburiae, and K. oxytoca was analysed regarding the score of each gene at codon positions 3–5 or 9–11. As an example, three genes (α, β, and γ) are shown. (C) Correlation matrix of the score of orthologous genes of E. coli, E. asburiae, and K. oxytoca. (D) The standard deviation score of each set of three orthologous genes was calculated and plotted as a frequency of distribution. (E) The top 500 genes with the best GFP score of E. coli, E. asburiae, and K. oxytoca were analysed regarding their orthology. The codon positions 3–5 or 9–11 were used to calculate the GFP score. As a control, a scramble list with 500 genes of each bacterium was used.