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Several validated miR-146a direct target genes are downregulated in miR-146a-transfected A549 cells. ΔΔCT qRT-PCR analysis indicated decreased mRNA levels of IRAK1, TRAF6, SMAD3, and EGFR in A549 cells transfected with 50 nM of synthetic miR-146a compared to levels in cells transfected with 50 nM of a non-targeting miRNA. RNA was isolated 48 hours after transfection. Gene expression was normalized to GAPDH mRNA. (*) p< 0.02, n= 3.
