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. 2019 Aug 23;16(12):1721–1732. doi: 10.1080/15476286.2019.1657351

Figure 3.

Figure 3.

Synthetic miR-146a transfection affects multiple steps of arachidonic acid metabolism. ΔΔCT qRT-PCR analysis was used to compare specific gene expression in A549 cells transfected with 50 nM of synthetic miR-146a to expression in cells transfected with 50 nM of a non-targeting miRNA. RNA was isolated 48 hours after transfection. Gene expression was normalized to GAPDH mRNA. (a) Analysis of mRNA levels of COX-1, COX-2, 5-LO, and FLAP, the proteins involved in the rate-limiting steps of arachidonic acid metabolism. (*) p< 0.05, n= 3. (b) Analysis of mRNA levels of mPGES-1 and TBXAS1, downstream synthases that produce bioactive PGE2 and TXA2, respectively. (*) p< 0.05, n= 3. (c) Analysis of mRNA levels of PTGER4, LTB4R, and TBXA2R, respective receptors for PGE2, LTB4, and TXA2. (*) p< 0.03, n= 3.