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. 2019 Aug 23;16(12):1721–1732. doi: 10.1080/15476286.2019.1657351

Figure 6.

Figure 6.

Multiple HuR target genes are downregulated in miR-146a-transfected A549 cells. (a) ΔΔCT qRT-PCR analysis indicated decreased mRNA levels of CCNA2, CCNB1, CCNE1, TGFB1, and HIF1A in A549 cells transfected with 50 nM of synthetic miR-146a compared to levels in cells transfected with 50 nM of non-targeting miRNA. RNA was isolated 48 hours after transfection. Gene expression was normalized to GAPDH mRNA. (*) p< 0.05, n= 3. (b) A549 cells were transfected with 50 nM of synthetic miRNAs, as in (a), with or without co-transfection of a HuR expression vector, pcDNA3.1(+)-HuR-FLAG. RNA was isolated 48 hours after transfection. ΔΔCT qRT-PCR analysis indicated significantly increased levels of CCNE1, TGFB1, and HIF1A in A549 cells where HuR was overexpressed, even in the presence of miR-146a. Gene expression was normalized to GAPDH mRNA. (*) p< 0.05, n= 3; (**) p< 0.01, n= 3.