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YAMAT-seq identified non-tRNA species containing non-genomic 3′-terminal CCA sequences.
(A) YAMAT-seq amplified cDNAs from MCF-7 cells were electrophoresed in 8% native polyacrylamide gel. The region designated with a line was subjected to gel-purification and Illumina sequencing.(B) Bioinformatics procedures to extract sequences of CCA-RNAs and NCCA-RNAs.(C, D) Sequences and secondary structures of the top 5 CCA-RNAs (C) and NCCA-RNAs (D).
