Figure 5.
The CCA-adding enzyme TRNT1 stabilizes the expression of CCA-RNAs.
(A) HeLa cells were transfected with control siRNA or siRNA targeting TRNT1. TRNT1 mRNA expression was efficiently knocked down by the targeted siRNA.(B) Total RNAs from control and TRNT1 KD cells were used for northern blots of (N)CCA-RNAs. Cytoplasmic tRNALysCUU and mitochondrial tRNAValUAC or other ncRNAs miR-16, U1/U2 snRNAs, and 5S rRNA were analyzed as control RNAs that are regulated, or not regulated, by TRNT1, respectively. Band quantifications are shown in Fig. S4A. A single asterisk: a non-specific cytoplasmic band; double asterisks: the bands of potential precursor RNAs of nccaR-1.