The expression of CCA-RNAs are responsive to cellular treatments.
HeLa cells were subjected to UV irradiation (UV), starvation cultured with EBSS, inhibition of mitochondrial respiratory complex I (rotenone) or IV (sodium azide), or inhibition of cell cycle (thymidine block), and the expression of CCA-RNAs were analyzed using northern blots. Mitochondrial tRNAValUAC and miR-16 were analyzed as control RNAs whose expressions were unaffected by the treatments. Band quantifications are shown in Fig. S4B. A single asterisk: a non-specific cytoplasmic band; double asterisks: the bands of potential precursor RNAs of nccaR-1.