Table 1.

Ty1 RNA mutants used in this study.

Mutant	Description of introduced mutations	Sequence of wild type mini Ty1 RNA and mutants
U16C mutant	Single nucleotide substitution in PAL1 sequence	15GUAUAU20	→ GCAUAU
PAL1 mutant	Substitution of PAL1 sequence with non palindromic sequence	13UAGUAUA19	→ CGCCCGC
PAL2 mutant	Substitution of PAL2 sequence with non palindromic sequence	24GUAUAC29	→ CGCCCG
PAL3 mutant	Substitution of PAL3 sequence with non palindromic sequence	423CCUGGG428	→ AAGAGG
IL3 mutant	Substitution of internal loop 3 sequence	65ACAAUUAU72	→ CCAACCAU
SL4 mutant	Substitution of SL4 apical loop sequence	291CCACAGAAU299	→ CCUCUCUAA
∆S1a mutant	Deletion of 5 nt within S1a pseudoknot stem	1GAGGA5	→ – – – – –