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. 2019 Aug 25;16(12):1733–1748. doi: 10.1080/15476286.2019.1657352

Figure 3.

Figure 3.

Overexpression of miR-142-3p inhibited endometrial cell metastasis and vascular endothelial cell tube formation. (a-c) Transwell migration and invasion assays to evaluate the metastasis of CRL-7566 cells treated with miR-142-3p mimics and inhibitor, *** P < 0.001. (d, e) Cell wound scratch assay to evaluate the migration of CRL-7566 cells transfected with miR-142-3p mimics and inhibitor, *** P < 0.001. (f-h) qRT-PCR, IF and Western blot analysis of VEGFA expression in CRL-7566 cells transfected with miR-142-3p mimics and inhibitor, *** P < 0.001. (I) ELISA analysis of VEGFA concentration in the culture medium of treated CRL-7566 cells, *** P < 0.001. (j, k) Tube formation assay to evaluate the culture medium gathered from treated CRL-7566 cell on the vascularization viability of HUVEC, * P < 0.05, ** P < 0.01.