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. 2019 Aug 25;16(12):1733–1748. doi: 10.1080/15476286.2019.1657352

Figure 9.

Figure 9.

Knockdown of KLF9 ameliorated the miR-142-3p inhibitor-mediated cell proliferation promotion and apoptosis inhibition. (a-c) qRT-PCR, Western blot and IF analysis of the expression of KLF9 in miR-142-3p inhibitor transfected CRL-7566 cells after KLF9-siRNA transfection, *** P < 0.001. (d, g) EdU staining analyse the cell proliferation viability of miR-142-3p inhibitor transfected CRL-7566 cell transfected with KLF9-siRNA, *** P < 0.001. (e, f) Flow cytometry to measure the effect of KLF9-siRNA on cell cycle in miR-142-3p inhibitor transfected CRL-7566 cells, *** P< 0.001. (h,i) Flow cytometry and Hoechst 33,258 staining methods were performed to analyse the effect of KLF9-siRNA on cell apoptosis in miR-142-3p inhibitor transfected CRL-7566 cells, ** P < 0.01. (j) Western blot analysis of VEGFA, Bcl-2, Bax, LC3B and p62 expression in miR-142-3p inhibitor transfected CRL-7566 cells after KLF9-siRNA transfection.