Figure 10.

Effects of RFC3 on the regulation of proteins associated with epithelial-mesenchymal transition and the Wnt/β-catenin signaling pathway. (A) RFC3 overexpression plasmid and empty vector were transfected into the lung cancer A549 cell line. (B) RFC3-specific siRNA and non-specific control siRNA were transfected into the lung cancer H1299 cell line. Total protein was extracted and subjected to immunoblotting of Wnt1, p-GSK3-β (Ser9), GSK3-β, β-catenin, c-MYC, E-cadherin, N-cadherin and Vimentin. Actin was used as a loading control. (A and B) Representative image data of immunoblotting, semi-quantitative representation of protein expression and ratios of p-GSK3-β/GSK3-β from three separate experiments. ^*P<0.05, ^**P<0.01. GSK3, glycogen synthase kinase 3; p-, phosphorylated; RFC3, replication factor C subunit 3; siRNA, small interfering RNA.