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Secretion of TGFβ1, IL-8, and NO was assessed by ELISA. MCF7 cells were cultured for 24, 48, and 72 h under nutrient deprived conditions. The levels of TGFβ1, IL-8, and NO in the medium following nutrient deprivation were measured by ELISA. The results were expressed as mean ± standard deviation (SD). (*P< 0.05, **P< 0.01)
