Table 3.

PCR conditions for tetra-ARMS PCR genotyping of rs1801131 A/C and rs1801133 T/C polymorphisms.

Polymorphism	Cycle	Temperature (ºC)	Time (minutes)	Number of cycles
rs1801133 T/C	Initial Denaturation	95	5:00	1
	Denaturation	95	0:30	35
	Annealing	66	0:30
	Extension	72	0:45
	Final extension	72	05:00	1
rs1801131 A/C	Initial Denaturation	95	05:00	1
	Denaturation	95	0:30	35
	Annealing	54	0:30
	Extension	72	0:45
	Final extension	72	05:00	1