Skip to main content
. 2019 Oct 21;44(6):2077–2090. doi: 10.3892/ijmm.2019.4379

Figure 1.

Figure 1

Pulmonary levels of SPHK1 and S1P are upregulated in the two-hit model of ventilator-associated lung injury. mRNA expression levels of (A) SPHK1 and (B) SPHK2 in ventilated mouse lung tissues. Data were obtained from the following Gene Expression Omnibus datasets: GSE58169, GSE29920 and GSE9208. Data are presented as the mean ± SEM. (C) mRNA expression levels of SPHK1 were detected by reverse transcription-quantitative PCR. Protein expression of SPHK1 was normalized to β-actin. (D) Representative protein bands are shown. (E) At the end of the experiment, blood was collected from the heart, and the supernatant was removed after centrifugation. Alterations in the levels of S1P were detected by ELISA. Data are presented as the mean ± SEM (n=7). &&P<0.01 vs. Control group. *P<0.05, **P<0.01 vs. LPS or ventilated groups. ##P<0.01 vs. Ventilated + LPS group. LPS, lipopolysaccharide; S1P, sphingosine 1 phosphate; SPHK, sphingosine kinase.