Figure 4.

Effect of SPHK1 inhibitor on the RhoA/ROCK pathway in the two-hit model of ventilator-associated lung injury. Levels of (A) GTP-RhoA, (B) ROCK1 and (C) p-MYTP1 were determined by western blot analysis. GTP-RhoA levels were normalized to total RhoA expression, and the p-MYTP1/total-MYTP1 ratio was evaluated. The representative protein bands were shown. Data are presented as the mean ± SEM (n=7). ^**P<0.01 vs. LPS or ventilated groups; ^#P<0.05, ^##P<0.01 vs. Ventilated + LPS group. LPS, lipopolysaccharide; MYTP1, myosin phosphatase target subunit 1; p, phosphorylated; ROCK, Rho-associated coiled-coil forming protein kinase; RhoA, Ras homolog family member A; SPHK, sphingosine kinase.