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. 2019 Oct 21;44(6):2077–2090. doi: 10.3892/ijmm.2019.4379

Figure 5.

Figure 5

Effects of SPHK1 inhibitor on the RhoA/ROCK pathway in mouse lung vascular endothelial cells. Cells were collected to analyze (A) the protein expression levels of GTP-RhoA, (B) ROCK1 and (C) p-MYTP1 expression. GTP-RhoA levels were normalized to total RhoA expression and the p-MYTP1/total-MYTP1 ratio was evaluated. The representative protein bands were shown. Data are presented as the mean ± SEM (n=4). *P<0.05, **P<0.01 vs. LPS or stretched groups; #P<0.05, ##P<0.01 vs. Stretched + LPS group. LPS, lipopolysaccharide; MYTP1, myosin phosphatase target subunit 1; p, phosphory-lated; ROCK, Rho-associated coiled-coil forming protein kinase; RhoA, Ras homolog family member A; SPHK, sphingosine kinase.