Table III.

Sequences of mouse primers used for RT-qPCR.

Primer name	Primer sequence	Product length (bp)
M-GAPDH
Forward	5′-TGAAGGGTGGAGCCAAAAG-3′	227
Reverse	5′-AGTCTTCTGGGTGGCAGTGAT-3′
M-SREBP-1c
Forward	5′-ACAGACAAACTGCCCATCCA-3′	223
Reverse	5′-GCAAGAAGCGGATGTAGTCG-3′
M-FAS
Forward	5′-ATCTGGGCTGTCCTGCCTCT-3′	116
Reverse	5′-TTATCAGTTTCACGAACCCGC-3′
M-AMPKα
Forward	5′-GATGATGACCATGTGCCAACTC-3′	270
Reverse	5′-CTCCGAACACTCGAACTTCTCAC-3′
M-CPT1α
Forward	5′-CATGATTGCAAAGATCAATCGG-3′	141
Reverse	5′-AGCACCTTCAGCGAGTAGCG-3′
U6
RT-primer	5′-AACGCTTCACGAATTTGCGT-3′
Forward	5′-CTCGCTTCGGCAGCACAT-3′
Reverse	5′-AACGCTTCACGAATTTGCGT-3′
mmu-miR-21
RT-primer	5'-CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTCAACATC-3′
Forward	5′-ACGGCTTATCAGACTGATGTTGA-3′
Reverse	5′-CTCAACTGGTGTCGTGGAGTC-3′

GAPDH, glyceraldehyde-3-phosphate dehydrogenase; SREBP1-c, sterol regulatory element-binding transcription factor 1C; FAS, fatty acid synthase; AMPKα, adenosine 5-monophosphate (AMP)-activated protein kinase α; CPT1α, carnitine palmitoyl transferase 1α; miR-21, microRNA-21.