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. 2019 Oct 9;8:e47021. doi: 10.7554/eLife.47021

Figure 3. Difference in CF signals between the lateral and medial Crus II.

(A) Extracted ROIs representing PC dendrites that are pseudocolored overlaid on the tdTomato image around a 7+/6− (top) and a 5a+/4b− (bottom) boundary. Scale bars, 40 μm. (B) Single ROI trial-averaged traces for 7+ and 6− (top) and 5a+ and 4b− (bottom) compartments from the data in panel (A). Thick lines represent ROI-averaged traces. (C) Percentage of responsive ROIs during the early response window (0 to 0.5 s from cue onset) in an AldC compartment per session for each trial type pooled across all the lateral AldC+ or medial AldC+ compartments (n = 12 and 13 mice, 29 and 50 imaging sessions, respectively). Colored dots represent means. Colors represent trial types (hit, magenta; FA, green; CR, blue). (D) Same as panel (C) but for all the lateral AldC− or medial AldC− compartments (n = 15 and 9 mice, 44 and 35 imaging sessions, respectively). (E) Schematic diagram of the left Crus II showing a functional grouping of AldC compartments. The dashed line represents a boundary between the lateral and medial Crus II. (C, D) Two-way ANOVA on ranks with repeated measures followed by post-hoc Tukey’s test: *p<0.05, **p<0.01, ***p<0.001.

Figure 3—source data 1. Datasets used to create Figure 3.
elife-47021-fig3-data1.mat (1.5KB, mat)
DOI: 10.7554/eLife.47021.020

Figure 3.

Figure 3—figure supplement 1. Mediolateral separation of task-related CF signals in the Crus II.

Figure 3—figure supplement 1.

(A) Extracted ROIs representing PC dendrites around the example 7+/6−, 6−/6+, 6+/5−, 5−/5+, 5+/5a−, 5a−/5a+, and 5a+/4b− boundaries that are pseudocolored and overlaid on the tdTomato image. Scale bars, 40 μm. (B) Single ROI trial-averaged traces for all the AldC compartments from the data in panel (A). Thick lines represent ROI-averaged traces. (C) Z-scored trial-averaged ΔF/F for each compartment averaged across sessions and mice, aligned from lateral to medial separately for AldC+ and AldC− compartments (n = 5, 3, 8, 9, 8, 3, and two mice, and 12, 4, 13, 15, 16, 13 and 6 sessions for 7+/6−, 6−/6+, 6+/5−, 5−/5+, 5+/5a−, 5a−/5a+ and 5a+/4b− boundaries, respectively). Vertical dashed lines delineate the lateral and medial Crus II. Horizontal dashed lines represent cue onset.
Figure 3—figure supplement 1—source data 1. Datasets used to create Figure 3—figure supplement 1.
elife-47021-fig3-figsupp1-data1.mat (1.4KB, mat)
DOI: 10.7554/eLife.47021.018
Figure 3—figure supplement 2. Difference in secondary CF signals between the lateral and medial Crus II.

Figure 3—figure supplement 2.

(A) Percentage of responsive ROIs during the secondary response window (0.5 to 1 s from cue onset) in a compartment per session for each trial type pooled across all the lateral AldC+ or medial AldC+ compartments (n = 12 and 13 mice, 29 and 50 imaging sessions, respectively). Colored dots represent means. Colors represent trial types (hit, magenta; FA, green; CR, blue). (B) Same as panel (A) but for all the lateral AldC− or medial AldC− compartments (n = 15 and 9 mice, 44 and 35 imaging sessions, respectively). (A, B) Two-way ANOVA on ranks with repeated measures followed by post-hoc Tukey’s test: **p<0.01, ***p<0.001.