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. 2019 Sep 12;47(20):10881–10893. doi: 10.1093/nar/gkz787

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — GLD-2 opposes CCR-4 and CCF-1 in the regulation of meiotic progression. (A) Diakinetic nuclei are absent from gonads lacking GLD-2 but form upon the additional removal of certain deadenylase activities. Percentage of germ lines in which diakinetic nuclei can be detected in the proximal part as judged by DAPI staining. (B) Examples of germ cell nuclei in the proximal part of extruded gonads from indicated genetic backgrounds. DAPI staining (purple in merge) marks DNA; NPC = Nuclear pore complex (green in merge). (C) Extruded gonads of indicated genetic backgrounds stained for the oocyte marker proteins OMA-1/2 (oo, red in merge); and DAPI to reveal chromatin morphologies (green in merge). Scale bars = 10 μm.