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. 2019 Sep 6;47(20):10842–10851. doi: 10.1093/nar/gkz765

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — Conformational Switching of Prp5^core Requires ATP and RNA. (A) Schematic of the smFRET assay. (B) Top: Cy3 (green) and Cy5 (red) fluorescence trajectories from a single molecule of Prp5^core. Bottom: Calculated E_FRET from the shown trajectories. (C) Histogram of E_FRET values obtained for Prp5^core (grey boxes) and a fit of the data (blue line). (D) Data from a single molecule of Prp5^core as in panel (B) except in the presence of poly(A) RNA and ATP. (E) Histogram of E_FRET values obtained for Prp5^core in the presence of poly(A) RNA and ATP as in panel (C) and a fit of the data (red line). n and m represent the number of individual molecules and E_FRET data points, respectively, used to construct the histogram. Data fit results are listed in Supplementary Table S4.