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. 2019 Sep 6;47(20):10842–10851. doi: 10.1093/nar/gkz765

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Impact of E235A and SAT-motif Mutations on Prp5^core Dynamics. (A–C) Histograms of E_FRET values obtained for Prp5^core-GAG (A), -TAG (B) and -GAR (C) in the presence of poly(A) RNA and ATP (gray boxes). n and m represent the number of individual molecules and E_FRET data points, respectively, used to construct the histogram. (D) Calculated K_close values and ATPase rates in the presence of poly(A) RNA obtained for Prp5^core and the indicated mutants. Histogram fit results, calculated lifetimes, and K_close values are listed in Supplementary Table S5. ATPase assays were carried out in triplicate and the shown value represents the average ±SD.