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AID-catalyzed dC NTS and TS deamination spectra on IGHV3-23*01 during transcription with T7 Pol. An IGHV3-23*01 dsDNA substrate, containing a T7 promoter, was transcribed in the presence of AID and analyzed by MDS. The C → T mutation rate is shown at each dC site in the IgV target sequence (5′WRC hot-motifs, red bars, 5′SYC cold-motifs, blue bars; all other motifs containing a C site, green bars).
