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. 2019 Sep 23;47(20):10693–10705. doi: 10.1093/nar/gkz792

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 9. — The absence of polar and aromatic steric filter in PolIV results in a drastic reduction in sugar selectivity. (A) Primer extension assays showed that the mutant version of PolIV without the steric and polar filter exhibits a 50-fold enhancement in rCTP incorporation. The error bars indicate standard deviation (n = 3). (B) Primer extension assays showed that the absence of the steric and polar filters did not affect the ability of PolIV to incorporate dCTP. The error bars indicate standard deviation (n = 3). (C) The PolIV-F13A+T43C_DNA(dG):rCTP (peach) structure superimposed with PolIV-F13A+T43C_DNA(dG):dCTP (violet) structure with an RMSD of 0.26 Å. The position and conformation of the dCTP and rCTP in the two structures are identical.