Table 1.

Steady-state kinetics of rCTP incorporation by MsDpo4-WT and MsDpo4-L14Y

Protein	k cat a (min−1)	K m a (μM)	k cat/Kma (μM−1 min−1)
MsDpo4-WT	0.66 ± 0.07	55.3 ± 4.0	0.01 ± 0.001
MsDpo4-L14Y	0.1 ± 0.01	59.54 ± 13.4	0.0016 ± 0.0003

^aThe numbers after ± denote standard deviation (n = 3).