Figure 6.

Has1 can be recruited to 18S and 25S regions in pre-rRNA independently. (A, C) Schematic representation of the rDNA truncations used. (B, D) RNA co-purified with Has1-FTP was analyzed by northern blotting using probes against the MS2 tag or 18S-tag respectively. Probing for SCR1 RNA was used as the purification control. Lanes I - 1% of the input, lanes E - total eluted RNA purified with Has1-FTP. The black arrows indicate the full-length transcripts of each truncation. The white arrows indicate transcripts partially processed by cleavage at sites A0/A1, A2 and C2 respectively.