Figure 2.

Converting human ADAR3 into an active deaminase by introducing five mutations. (A) Evaluation of RNA editing activity of hADAR3-D mutants using the fluorescent reporter assay. WT is the wild type hADAR3-D. M1, M2 and M3 are hADAR3-D mutants introduced with mutations as shown. F/F0 is the ratio of sample fluorescence divided by negative control (inactive mutant) fluorescence. Error bar indicates SD, n ≥ 3. (B) Editing of the catalytically active ADAR3 mutant M3 on BDF200 RNA substrate at t = 0 min and t = 60 min. The editing site is shown by an arrow. (C) Fraction edited by ADAR3-D M3 in the BDF2 model substrate RNA as a function of time. Error bar means SD. n ≥ 3.