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. Author manuscript; available in PMC: 2019 Nov 11.
Published in final edited form as: Nature. 2018 Feb 28;555(7695):265–268. doi: 10.1038/nature25787

Figure 2. Origin unwinding takes place in two steps.

Figure 2

a, active CMG was assembled on a radiolabelled 616 bp ARS1 circle without RPA for 40 min and products separated on a native bis-polyacrylamide gel. b, as in a, except all firing factors were omitted. MCM loading does not occur in ATPγS. c, as in a, with proteins omitted as indicated.