Table 2.
Isolation of I-OnuI variants on substrates with substitutions in A+3
| Library | Substrate | Substitutions | Independent isolations |
|---|---|---|---|
| 1NNS | A+3 | K227K | 2 |
| K227S | 2 | ||
| A+3G | none | none | |
| A+3T | none | none | |
| 3NNS | A+3 | K227G/W234/D236S | 2 |
| K227G/W234/D236V | 2 | ||
| K227R/W234/D236 | 1 | ||
| A+3G | K227Y/W234/D236A | 5 | |
| A+3T | K227/W234/D236E | 15 |
The 1NNS library refers to position 227, while 3NNS library refers to positions 227, 234 and 236. All positions are reported for the 3NNS library regardless of whether a substitution was found at each position.