Table 1.

ITC analysis of 2′-dG and rG binding to purine family riboswitches

Riboswitch	Class	K D,2′-dG (nM)	K D,rG (nM)	K rel (rG/2′-dG)
env1	2′-dG-II	34 ± 1	89 ± 10	2.6
B. subtilis xpt	G/A	7700 ± 100	36 000 ± 1000	2.6
env30	2′-dG-II	2400 ± 900	36 000 ± 1000	15
M. florum I-A	2′-dG-I	190 ± 10	5000 ± 600a	27
xpt GdG 5B a	synthetic	350 ± 100	10 000 ± 100	29
env6	2′-dG-II	88 ± 3	3000 ± 200	35
env23	2′-dG-II	290 ± 50	51 000 ± 4000	180

^aData from reference (11).