Table 2.
The thermodynamic parameters of RNA and RNA-CMBL complexes
| Average of curve fit | T M −1 versus log CT plots | |||||||
|---|---|---|---|---|---|---|---|---|
| –ΔH (kcal/mol) | –ΔS (eu) | –ΔG37 (kcal/mol) | T M a(°C) | –ΔH (kcal/mol) | –ΔS (eu) | –ΔG37 (kcal/mol) | T M a (°C) | |
| (GCAGCAGC)2 | 39.1±4.6 | 109.5±15.4 | 5.2±0.1 | 33.1 | 38.6±2.0 | 107.7±6.7 | 5.2±0.1 | 33.0 |
| b(GCAGCAGC)2-CMBL3a | 68.0±2.4 | 191.1±7.3 | 8.7±0.6 | 51.5 | 42.2±2.4 | 110.8±7.6 | 7.8±0.1 | 53.6 |
| b(GCAGCAGC)2-CMBL3b | 55.0±10.0 | 157.8±31.0 | 6.1±0.5 | 39.3 | 34.6±4.6 | 92.1±15.0 | 6.0±0.3 | 40.1 |
| b(GCAGCAGC)2-CMBL4 | 57.2±6.7 | 163.4±21.2 | 6.5±0.2 | 41.4 | 61.6±4.4 | 177.7±14.3 | 6.5±0.1 | 41.4 |
aMelting point was calculated at the oligomer concentration of 10−4 M.
bThermodynamic parameters were calculated assuming saturating levels of ligand concentration.