Figure 3.

Extrafollicular plasma cell differentiation is impaired in the absence of Hdac3. (A) 5 or 10 days post-immunization with SRBCs, spleens were dissociated and analyzed for the presence of CD138⁺B220^lo plasma cells. (B) Graph depicts quantitation of the cohort from A. (C) Mice were immunized with NP-CGG. Eight days post-immunization, serum was harvested and serial serum dilutions were used in ELISAs detecting the indicated immunoglobulin using NP(31)-BSA coated plates. (D) Serum from C was used to detect IgG1 binding to NP(7)-BSA coated plates. (E) Splenic B cells were isolated and stimulated ex vivo with αCD40 and IL-4 for 4 days to induce immunoglobulin class switching to IgG1. Right panel shows quantification of populations identified from multiple mice/genotype. (F) Splenic B cells were isolated and stimulated ex vivo with LPS for 4 days to induce immunoglobulin class switching to IgG3. Right panel shows quantification of populations identified in from multiple mice/genotype.