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. 2019 Oct 5;47(20):10612–10627. doi: 10.1093/nar/gkz816

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Altered germinal centers in the absence of Hdac3. (A) Mice were immunized with sheep red blood cells (SRBCs). 5 and 10 days post-immunization, spleens were harvested and analyzed by flow cytometry for the presence of B220⁺IgD^loFas⁺ germinal center B cells. Graphical representation of GC B cell populations as a percentage of total splenic B cells from at least 6 mice per group is presented at the right. (B) Spleens were isolated from SRBC-injected mice ten days following immunization and sections were stained with PNA to identify germinal center B cells. (C) Ki67 staining was performed on 10 dpi spleens to identify proliferating cells. (D) Immunized mice were injected with BrdU 2 h prior to tissue harvest. Germinal center B cells were analyzed by flow cytometry for BrdU incorporation. (E) Splenocytes were labeled with annexin V and analyzed by flow cytometry. The percentage of B220⁺IgD^loFas⁺ Annexin V⁺ splenocytes are depicted.