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. 2019 Oct 10;47(20):10771–10787. doi: 10.1093/nar/gkz875

Figure 5.

Figure 5.

Ectopic expression of the distal-specific NQO1 isoform in the presence of Star-PAP reverses molecular events of hypertrophy in cardiomyocyte. (A) qRT-PCR analysis of the distribution of NQO1 mRNA expressed as percent of total mRNA relative to GAPDH mRNA in each fraction across all fractions in the sucrose density gradient. Error bar represents SEM of n = 3 independent experiments (P-values <0.04 of NQO1 in all the fractions). (B, C) Phase contrast (PC) and IF imaging of phalloidin stained H9c2 cells after treatment with isoproterenol in the presence of ectopic expression of different constructs of NQO1 isoforms and Star-PAP as indicated, and quantification of cell surface area of the phalloidin stained H9c2 cells (P value <0.01 for all conditions). Error bar represents SEM. (D) Western blot analysis of Star-PAP, NQO1, PAPα, and SERCA2A after treatment with isoproterenol in the presence of ectopic expression of different constructs of FLAG-NQO1 and Star-PAP as indicated. Each blot is representative of 3 independent experiments. (E) qRT-PCR analysis of hypertrophic markers (ANP, SERCA2A and β-MHC) in H9c2 cell line under conditions as indicated. (P-values ANP <0.05, SERCA2A <0.04, b-MHC <0.01). Error bar represents SEM, n = 3 independent experiments.