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. 2019;36(2):119–123. doi: 10.5511/plantbiotechnology.19.0220b

Figure 1. Transient GFP expression in leaves (A, C) and seedpods (B, D) of Lotus japonicus Miyakojima MG-20 (A, B) and Gifu B-129 (C, D) infiltrated with Agrobacterium tumefaciens containing pBYR2HS-EGFP. Leaves of two-month-old plants and about 2-cm length of green seedpods were used for infiltration. GFP was stimulated using a hand-held blue LED lamp, and emission was observed using an ultraviolet-absorbing filter (Fujifilm SC-52). No signal was detected in leaves and fruits of the negative control (NT). Autofluorescence was observed as red fluorescence due to excitation of chlorophyll by blue light. The size bar indicates 1 cm. Five or more samples were used for biological replication.

Figure 1. Transient GFP expression in leaves (A, C) and seedpods (B, D) of Lotus japonicus Miyakojima MG-20 (A, B) and Gifu B-129 (C, D) infiltrated with Agrobacterium tumefaciens containing pBYR2HS-EGFP. Leaves of two-month-old plants and about 2-cm length of green seedpods were used for infiltration. GFP was stimulated using a hand-held blue LED lamp, and emission was observed using an ultraviolet-absorbing filter (Fujifilm SC-52). No signal was detected in leaves and fruits of the negative control (NT). Autofluorescence was observed as red fluorescence due to excitation of chlorophyll by blue light. The size bar indicates 1 cm. Five or more samples were used for biological replication.