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. 2019 Nov 7;12:9341–9350. doi: 10.2147/OTT.S221040

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© 2019 Tang et al.

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Lentiviral gene transfer combined with αCD3/αCD28 antibody-mediated activation of T cells permits the generation of large numbers of αLMP1-specific chimeric antigen receptor (CAR⁺) T cells. (A) Schematic diagram showing the αLMP1-specific CAR used in this study. (B) αLMP1-specific CAR surface expression in primary human CD3+ T cells by detecting ZsGreen expression. Expression was examined 8 days after transduction with the indicated CAR-encoding lentiviral vector at an MOI of ~50. (C) CD3ζ expression analyzed by Western blot analysis of αLMP1-specific CART cells. (D) In vitro expansion of CD3+ T cells following activation with αCD3/αCD28 antibodies and transduction of the indicated CAR on day 2. Data are representative of 3–5 independent experiments, PBMC isolated from different individuals.