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. 2019 Nov 7;11:9371–9378. doi: 10.2147/CMAR.S220434

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© 2019 Yao et al.

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ASB16-AS1 regulates miR-1827/FZD4/Wnt/β-catenin pathway. (A) Strategies for construction of wild-type (WT) or mutant (MUT) ASB16-AS1and FZD4 luciferase reporter vectors. (B and C) Luciferase reporter assay using ASB16-AS1and FZD4 luciferase reporter vectors in Huh7 cells. (D) Pulldown assay indicated miR-1827 interacted with ASB16-AS1. (E) RIP assay showed miR-1827 interacted with FZD4 mRNA in Huh7 cells. (F) ASB16-AS1 knockdown increased miR-1827 level. (G) Expression of FZD4 was tested after transfection with indicated vectors. (H) Target genes (CCND1, CCND2, MYC and SOX4) of Wnt/β-catenin pathway were analyzed by qRT-PCR in Huh7 cells. (I) FZD4 expression was analyzed using UALCAN tool (http://ualcan.path.uab.edu/analysis.html). (J) Relative expression of FZD4 was analyzed by qRT-PCR in HCC tissues. *p<0.05.