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. 2019 Nov 5;10:2472. doi: 10.3389/fimmu.2019.02472

Figure 1.

Figure 1

Design and expression of DTT adjuvant-based neoantigen vaccine. (A) A schematic diagram of DTT-neoAg and DTT-wtAg. M1, M2, M3, and M4 are the mutation peptide of Pi4k2b, Ddb1, Pcdhga11 and Atp11a, respectively. W1, W2, W3, and W4 are the corresponding wild type peptides. Each mutation peptide is 27 amino acid residues in length with the mutated residue positioned in the central (position 14). The horizontal stripe box stands for the linker sequence (GGGGSGGGGS) and the diagonal stripe box stands for the linker sequence (GS). (B) 15% SDS-PAGE analysis of the purified recombinant proteins.