Table 2.
Primer sequences for genotyping transgenic mice.
| Genotype | Primer name | Nucleotide sequence (5′ → 3′) | Product size (bp) | Reference |
|---|---|---|---|---|
| CaMKII-Cre |
CaMKpro-F Cre |
ACGGGAACAGGGCGTTTCGGAGGTGGTTGC CTAATCGCCATCTTCCAGCAGG |
1300 | Riken BRC |
|
mLC3ex3GT mLC3ex4AG |
TGAGCGAGCTCATCAAGATAATCAGGT GTTAGCATTGAGCTGCAAGCGCCGTCT |
500 | Mizushima et al.53 | |
| GFAP-Cre |
oIMR1084 oIMR1085 |
GCGGTCTGGCAGTAAAAACTATC GTGAAACAGCATTGCTGTCACTT |
100 | Jackson Laboratory |
|
oIMR7338 oIMR7339 |
CTAGGCCACAGAATTGAAAGATCT GTAGGTGGAAATTCTAGCATCATCC |
324 | Jackson Laboratory | |
| Hrh1 Flox | loxP insert |
AACAACAATTCTTGGGCAGTG TGATGCTAGTTTGGAGGTAGTTAGG |
157/329 | This study |
|
Recom- bination |
GCCGCAAAACAATTGATAACTTC CTTTTTGGAGAAGGCGAGTG |
3244/1106 | This study |