Table 1.
Primer sequences and assay parameters of quantitative polymerase chain reactions for selected gene transcripts of warthogs.
| Gene | Primer sequence (5′-3′) | Reverse | Tannealing | Pig identity | E% | AF | R2 | IVA (%) | DM peak (°C) |
|---|---|---|---|---|---|---|---|---|---|
| Forward | |||||||||
| B2M | CTCACTGTCTGGCCTGGATG | GGCGGATGGAACCCAGATAC | 60 °C | F: 100%; R: 100% | 103 | 2.03 | 0.99 | 0.5 | 82.5 |
| H3F3A | AAACAGATCTGCGCTTCCAG | ACGTTTGGCATGGATAGCAC | 60 °C | F: 100%; R: 100% | 105 | 2.05 | 0.99 | 0.4 | 80.5 |
| LDHA | TGCAACATGGCAGCCTTTTC | ACAACCAGCCTAGAGTTTGC | 62 °C | F: 100%; R: 100% | 111 | 2.11 | 0.99 | 0.5 | 79.5 |
| PPIA | TGAGTGGTTGGATGGCAAAC | TGGTCTTGCCATTCCTGGAC | 60 °C | F: 100%; R: 100% | 114 | 2.14 | 0.99 | 0.7 | 77.5 |
| YWHAZ | TTCTGAACTCCCCAGAGAAAGC | GCGTGCTGTCTTTGTATGACTC | 62 °C | F: 100%; R: 100% | 109 | 2.09 | 0.99 | 0.3 | 77.5 |
| CXCL9 | TCATCTTCCTGACTCTGCTTGG | TGGATCATCCTTTGGCTGGTG | 62 °C | F: 95%; R: 100% | 97 | 1.97 | 0.99 | 0.8 | 77.0 |
| CXCL10 | CCCACATGTTGAGATCATTGCC | TCTCTCTGTGTTCGAGGAGATC | 60 °C | F: 77%; R: 100% | 108 | 2.08 | 0.99 | 0.5 | 76.5 |
| CXCL11 | AAAGCGGGAAGGTGTCTTTG | GGCATCTTCGTCCTTTATGTGC | 62 °C | F: 100%; R: 100% | 105 | 2.05 | 0.99 | 0.7 | 78.0 |
| IFNG | AGGCCATTCAAAGGAGCATG | AGTTCACTGATGGCTTTGCG | 60 °C | F: 100%; R: 100% | 115 | 2.15 | 0.98 | 1.0 | 78.5 |
| TNFA | GGCCCAAGGACTCAGATCATC | ATACCCACTCTGCCATTGGAG | 62 °C | F: 100%; R: 100% | 101 | 2.01 | 0.98 | 1.0 | 81.5 |
E%: Amplification efficiency; AF: Amplification factor; IVA: Intra-assay variability; DM: Derivative melt curve peak temperature; F: Forward primer identity; R: Reverse primer identity.