Table 1.
DNA melting temperatures (TM) of the C3TX and C3TXXX sequences (1 μM ss) with and without 1 equiv. Λ-Ru or Δ-Ru.
| Label | Sequence 5′ → 3′ | Native | Λ-Ru | Δ-Ru | ||
|---|---|---|---|---|---|---|
| TM | TM | ΔTM | TM | ΔTM | ||
| C3T3/333 | (CCCTTT)3CCC | 60.0 | 60.3 | +0.3 | 60.3 | +0.3 |
| C3T4 | (CCCTTTT)3CCC | 59.6 | 58.9 | −0.7 | 58.7 | −0.9 |
| C3T5 | (CCCTTTTT)3CCC | 51.7 | 51.4 | −0.3 | 51.4 | −0.3 |
| C3T6 | (CCCTTTTTT)3CCC | 47.0 | 48.4 | +1.4 | 48.2 | +1.2 |
| C3T7 | (CCCTTTTTTT)3CCC | 41.9 | 45.8 | +3.9 | 45.7 | +3.8 |
| C3T8/888 | (CCCTTTTTTTT)3CCC | 37.8 | 44.1 | +6.3 | 43.2 | +5.4 |
| C3T338 | CCCTTTCCCTTTCCCTTTTTTTTCCC | 49.1 | 50.8 | +1.7 | 50.5 | +1.4 |
| C3T383 | CCCTTTCCCTTTTTTTTCCCTTTCCC | 55.3 | 55.2 | −0.1 | 55.0 | −0.3 |
| C3T388 | CCCTTTCCCTTTTTTTTCCCTTTTTTTTCCC | 46.1 | 48.1 | +2.0 | 47.2 | +1.1 |
| C3T833 | CCCTTTTTTTTCCCTTTCCCTTTCCC | 49.5 | 51.0 | +1.5 | 50.8 | +1.3 |
| C3T838 | CCCTTTTTTTTCCCTTTCCCTTTTTTTTCCC | 39.8 | 45.7 | +6.0 | 45.6 | +5.9 |
| C3T883 | CCCTTTTTTTTCCCTTTTTTTTCCCTTTCCC | 46.6 | 48.8 | +2.2 | 47.8 | +1.2 |
Values are in degrees Celsius with a standard deviation of ±~0.1–0.6°C. Standard deviation for individual measurements are shown in Table S3.1.