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Graphical summary of secreted fragments for all C1r variants. All fragments of enzymatic active and inactive C1r variants in the supernatant of transfected HEK293T cells and detected on western blot by N- and C-terminal anti-C1r antibody are presented. Fragments presented in gray color indicate faint bands on western blot. Mutation p.S654A is marked by a star. Putative aggregate formation for CUB2 mutants is indicated.
