Fig. 1. Transcriptomic analysis reveals differential expression in male and female Sst^tdT interneurons.

a Representative confocal images of Sst^tdT mice with co-labeling for Pvalb. b Quantification of average number of Sst^tdT interneurons in each lamina of the medial PFC, percent represents proportion of Sst^tdT interneurons with co-expression of alternate marker. Here we show that 0–9.9% of Sst^tdT co-expressed Pvalb (n = 3/group; error bars indicate ± S.E.M.). c Schematic of procedure for FAC sorting interneuron subtypes followed by RNA-seq. d Volcano plot of genes differentially regulated between male and female Sst^tdT interneurons. Red dots indicate upregulated transcripts and blue dots represent downregulated transcripts. There are 1995 DEGs, n = 4–7 (FDR < 0.05). e Top five most significantly inhibited pathways (mostly downregulated) in female Sst^tdT interneurons vs. males. EIF2 signaling was the top inhibited pathway in females. f Mean FPKM of EIF2 signaling transcripts significantly lower in female Sst^tdT interneurons error bars indicate ± S.E.M. (FDR < 0.05). g Secondary confirmation of EIF2 signaling transcript expression levels in baseline male and female Sst^tdT interneurons by qPCR error bars indicate ± S.E.M. of log corrected Ct values (n = 3, Mann–Whitney-U, p < 0.01,)