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. 2019 Nov 11;10:5116. doi: 10.1038/s41467-019-13060-1

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© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — SOX9 protein is not detected in Runx1/Foxl2 DKO newborn XX gonads. Immunofluorescence for Sertoli cell markers DMRT1 and SOX9 and germ-cell marker TRA98 on consecutive sections in XY control (a, c) and XX Runx1/Foxl2 DKO gonads (b, d) at birth. Scale bar: 50 µm. At least three independent biological replicates were analyzed and the images presented are representative of all replicates