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. 2019 Nov 5;10:1421. doi: 10.3389/fpls.2019.01421

Figure 4.

Figure 4

Subcellular localization of green fluorescent protein (GFP)/Discosoma sp. red fluorescent protein (DsRed)-fusion organelle markers in protoplasts of stable transgenic rice plants. FP, fluorescent protein GFP or DsRed. Protoplasts of GFP-fusion (left panel) and DsRed-fusion (right panel) transgenic lines were examined by confocal microscopy. (A, B) GFP or DsRed alone; (C, D) ER marker AtWAK2-GFP-HDEL or AtWAK2-DsRed-HDEL; (E, F) Mitochondrial marker ScCOX4-GFP or ScCOX4-DsRed; (G) Golgi marker GFP-AtCCASP; (I, J) Peroxisome marker GFP-PTS1 or DsRed-PTS1; (L) cytoskeleton marker DsRed-mTn; (M, N) Plasmid marker CTP1-GFP or CTP1-DsRed; (O, P) Tonoplast marker Osγ-TIP-GFP or Osγ-TIP-DsRed; (Q, R) Plasma membrane marker GFP-OsPIP2 or DsRed-OsPIP2; (S) Nucleus marker GFP-OsH2B. N/A, No DsRed-AtCCASP- (H), GFP-mTn- (K), or DsRed-OsH2B-transgenic rice plants expressing obvious fluorescent signals (T) were obtained. Scale bars, 10 μm.