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. 2019 Nov 5;9:1175. doi: 10.3389/fonc.2019.01175

Figure 4.

Figure 4

FoxO1 knockdown promotes migration, invasion potential, and EMT in OSCC cells. (A) FoxO1 expression was detected by qRT-PCR and Western blot after transfected with FoxO1 shRNA in OSCC cells. (B) Images of the wound closure of monolayer Cal-27 and Tca8113 cells with FoxO1 knockdown at the time point of 0 and 24 h are presented on the left. Quantitative results are illustrated on the right. (C) The effect of FoxO1 knockdown on OSCC cells invasion were determined by Transwell assay with Matrigel, and the representative images are on the left. Quantitative results are illustrated on the right. (D) The effects of Cal-27-FoxO1 knockdown on expressions of EMT markers, E-cadherin, N-cadherin, β-catenin, and Vimentin, were measured using qRT-PCR and Western blot. And the effects of Cal-27-sh-2-STAT3/sh-1-FoxO1 on expressions of EMT markers, E-cadherin, N-cadherin, β-catenin, and Vimentin, were measured using qRT-PCR compared with those of sh-2-STAT3/scramble group. β-actin was used as a loading control. (E) Flow cytometry was used to examine the percentage of apoptotic cells in Cal-27 cells with FoxO1 knockdown and scramble control cells. All assays were carried out in triplicate. Results were shown as means ± SD. *P < 0.05.