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. 2019 Nov 5;9:1175. doi: 10.3389/fonc.2019.01175

Figure 5.

Figure 5

FoxO1 knockdown promotes aerobic glycolysis in OSCC cells. (A) Glucose consumption assay was used to detect the glucose consumption in Cal-27 and Tca8113 cells transfected with scramble, sh-1-FoxO1, sh-2-STAT3/scramble, or sh-2-STAT3/sh-1-FoxO1. The data showed that down-regulation of FoxO1, or both FoxO1 and STAT3 led to higher glucose consumption in Cal-27 and Tca8113 cells. (B) Lactate production assay was used to detect the lactate production in Cal-27 and Tca8113 cells transfected with scramble, sh-1-FoxO1, sh-2-STAT3/scramble, or sh-2-STAT3/sh-1-FoxO1. The data showed that down-regulation of FoxO1, or both FoxO1 and STAT3 led to higher lactate production in Cal-27 and Tca8113 cells. (C) PKM2 mRNA levels in Cal-27 and Tca8113 cells transfected with scramble, sh-1-FoxO1, sh-2-STAT3/scramble, or sh-2-STAT3/sh-1-FoxO1 were examined by qRT-PCR. (D) LDHA mRNA levels in Cal-27 and Tca8113 cells transfected with scramble, sh-1-FoxO1, sh-2-STAT3/scramble, or sh-2-STAT3/sh-1-FoxO1 were examined by qRT-PCR. Each assay was carried out in triplicate. Results were shown as means ± SD. *P < 0.05.