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. 2019 Nov 5;10:1380. doi: 10.3389/fphys.2019.01380

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Copyright © 2019 Montanari, Boschi and Colitti.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cell culture treatment protocol. Cells were grown until 90% (X9) or 100% (3T3-L1) confluence. Differentiation lasted 3 days. From day 3, cells were incubated with maintenance medium supplemented with different treatments lasting for 1 (4d) or 5 days (8d) (sampling times).